| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Fusobacterium necrophorum | CTGGGAACGGAAGCTTTTGG | TAATGCCTCCAAGCGGTCC | 59.40 | 59.78 | 219 | 182 |
100.00%
|
50.00%
|
| Primer 2 | Fusobacterium necrophorum | TGGGAACGGAAGCTTTTGG | TAATGCCTCCAAGCGGTCC | 58.27 | 59.78 | 218 | 182 |
100.00%
|
50.00%
|
| Primer 3 | Fusobacterium necrophorum | TGCAGCTCTTCCGTCTTGG | ACATCTTGCTCCGGTCTCC | 60.00 | 59.10 | 234 | 160 |
100.00%
|
50.00%
|
| Primer 4 | Fusobacterium necrophorum | GGGAACGGAAGCTTTTGGAG | TAATGCCTCCAAGCGGTCC | 59.12 | 59.78 | 217 | 148 |
100.00%
|
50.00%
|
| Primer 5 | Fusobacterium necrophorum | AGGACTGGATGTCGGGTGT | CCAACTCCTTCACAGCTTGC | 60.23 | 59.40 | 156 | 148 |
100.00%
|
50.00%
|
| Primer 6 | Fusobacterium necrophorum | ATGATGACGTAGCGGAGGC | TCTTCTCCTGCCAGCTCTC | 59.64 | 58.41 | 196 | 143 |
100.00%
|
50.00%
|
| Primer 7 | Fusobacterium necrophorum | ATGATGACGTAGCGGAGGC | CGTCTTCTTCTCCTGCCAGC | 59.64 | 60.74 | 201 | 143 |
100.00%
|
50.00%
|
| Primer 8 | Fusobacterium necrophorum | ATGATGACGTAGCGGAGGC | TCGTCTTCTTCTCCTGCCAG | 59.64 | 59.11 | 202 | 143 |
100.00%
|
50.00%
|
| Primer 9 | Fusobacterium necrophorum | ATGATGACGTAGCGGAGGC | TTCTTCTCCTGCCAGCTCTC | 59.64 | 59.10 | 197 | 143 |
100.00%
|
50.00%
|
| Primer 10 | Fusobacterium necrophorum | ATGATGACGTAGCGGAGGC | TCTTCTTCTCCTGCCAGCTC | 59.64 | 59.10 | 199 | 143 |
100.00%
|
50.00%
|